Novel vaccine regimen elicits strong airway immune responses and control of respiratory syncytial virus in nonhuman primates.

ABSTRACT:

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Induction of long-lasting immunity against viral respiratory tract infections remains an elusive goal. Using a nonhuman primate model of human respiratory syncytial virus (hRSV) infection, we compared mucosal and systemic immune responses induced by different DNA delivery approaches to a novel parenteral DNA prime-tonsillar adenoviral vector booster immunization regimen. Intramuscular (i.m.) electroporation (EP) of a DNA vaccine encoding the fusion protein of hRSV induced stronger systemic immune responses than intradermal EP, tattoo immunization, and conventional i.m. DNA injection. A single EP i.m., followed by two atraumatic tonsillar immunizations with the adenoviral vector, elicited strong systemic immune responses, an unique persistent CD4(+) and CD8(+) T cell resp

SUBMITTER: Grunwald T 

PROVIDER: S-EPMC3993754 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature