Single-cell and deep sequencing of IgG-switched macaque B cells reveal a diverse Ig repertoire following immunization.

ABSTRACT: The nonhuman primate model is important for preclinical evaluation of prophylactic and therapeutic intervention strategies. The recent description of the rhesus macaque germline Ig loci and establishment of a database of germline gene segments offer improved opportunities to delineate Ig gene usage in the overall B cell repertoire as well as in response to vaccination. We applied 454-pyrosequencing and single-cell RT-PCR of bulk and sorted memory B cells, respectively, to investigate IGHV gene segment expression in rhesus macaques. The two methods gave remarkably concordant results and identified groups of gene segments that are frequently or rarely used. We further examined the VH repertoire of Ag-specific memory B cells induced by immunization with recombinant HIV-1 envelope glycoprotein

SUBMITTER: Sundling C 

PROVIDER: S-EPMC3993955 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature