Interferon-induced transmembrane protein 1 regulates endothelial lumen formation during angiogenesis.

ABSTRACT:

Objective

It is well established that angiogenesis is a complex and coordinated multistep process. However, there remains a lack of information about the genes that regulate individual stages of vessel formation. Here, we aimed to define the role of human interferon-induced transmembrane protein 1 (IFITM1) during blood vessel formation.

Approach and results

We identified IFITM1 in a microarray screen for genes differentially regulated by endothelial cells (ECs) during an in vitro angiogenesis assay and found that IFITM1 expression was strongly induced as ECs sprouted and formed lumens. We showed by immunohistochemistry that human IFITM1 was expressed by stable blood vessels in multiple organs. siRNA-mediated knockdown of IFITM1 expression spared EC sprouting but completely d

SUBMITTER: Popson SA 

PROVIDER: S-EPMC3993998 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature