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Molecular characterization and expression analysis of chloroplast protein import components in tomato (Solanum lycopersicum).


ABSTRACT: The translocon at the outer envelope membrane of chloroplasts (Toc) mediates the recognition and initial import into the organelle of thousands of nucleus-encoded proteins. These proteins are translated in the cytosol as precursor proteins with cleavable amino-terminal targeting sequences called transit peptides. The majority of the known Toc components that mediate chloroplast protein import were originally identified in pea, and more recently have been studied most extensively in Arabidopsis. With the completion of the tomato genome sequencing project, it is now possible to identify putative homologues of the chloroplast import components in tomato. In the work reported here, the Toc GTPase cDNAs from tomato were identified, cloned and analyzed. The analysis revealed that there are four

SUBMITTER: Yan J 

PROVIDER: S-EPMC3994019 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature