Entamoeba histolytica acetyl-CoA synthetase: biomarker of acute amoebic liver abscess.

ABSTRACT:

Objective

To characterize the Entamoeba histolytica (E. histolytica) antigen(s) recognized by moribound amoebic liver abscess hamsters.

Methods

Crude soluble antigen of E. histolytica was probed with sera of moribund hamsters in 1D- and 2D-Western blot analyses. The antigenic protein was then sent for tandem mass spectrometry analysis. The corresponding gene was cloned and expressed in Escherichia coli BL21-AI to produce the recombinant E. histolytica ADP-forming acetyl-CoA synthetase (EhACS) protein. A customised ELISA was developed to evaluate the sensitivity and specificity of the recombinant protein.

Results

A ∼75 kDa protein band with a pI value of 5.91-6.5 was found to be antigenic; and not detected by sera of hamsters in the control group. Tandem mass spectrome

SUBMITTER: Huat LB 

PROVIDER: S-EPMC3994353 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature