CXCR3-dependent recruitment and CCR6-mediated positioning of Th-17 cells in the inflamed liver.

ABSTRACT:

Background & aims

IL-17 secreting CD4 (Th17) and CD8 (Tc17) T cells have been implicated in immune-mediated liver diseases, but the molecular basis for their recruitment and positioning within the liver is unknown.

Methods

The phenotype and migratory behaviour of human liver-derived Th17 and Tc17 cells were investigated by flow cytometry and chemotaxis and flow-based adhesion assays. The recruitment of murine Th17 cells to the liver was studied in vivo using intra-vital microscopy.

Results

IL-17(+) T cells comprised 1-3% of the T cell infiltrate in inflammatory liver diseases and included both CD4 (Th17) and CD8 (Tc17) cells. They expressed RORC and the IL-23 receptor and included subsets that secreted IL-22 and interferon-γ. Th17 and Tc17 cells expressed high levels

SUBMITTER: Oo YH 

PROVIDER: S-EPMC3994510 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature