A phenotypic screening approach to identify anticancer compounds derived from marine fungi.

ABSTRACT: This study covers the isolation, testing, and identification of natural products with anticancer properties. Secondary metabolites were isolated from fungal strains originating from a variety of marine habitats. Strain culture protocols were optimized with respect to growth media composition and fermentation conditions. From these producers, isolated compounds were screened for their effect on the viability and proliferation of a subset of the NCI60 panel of cancer cell lines. Active compounds of interest were identified and selected for detailed assessments and structural elucidation using nuclear magnetic resonance. This revealed the majority of fungal-derived compounds represented known anticancer chemotypes, confirming the integrity of the process and the ability to identify suitable c

SUBMITTER: Ellinger B 

PROVIDER: S-EPMC3994914 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature