Sucrose stabilization of Respiratory Syncytial Virus (RSV) during nebulization and experimental infection.

ABSTRACT:

Background

Respiratory syncytial virus (RSV) is a common respiratory pathogen that can cause severe pneumonia. In vivo studies of RSV can be difficult due to variation in viral infection and disease severity in some animal models. Factors that may contribute to the variation are decreases in viral titer due to preparation and storage and method of virus administration. Nebulization is one method of RSV administration that provides even distribution of virus to all lung lobes; however, the exact quantity of the virus killed by nebulization is not defined. To test the hypothesis that sucrose enhances RSV stability and infectivity, a series of in vitro experiments were conducted with RSV strain Memphis 37 stored at varying concentrations (0%, 3%, 5%, 8%, 10%, 15%, and 20%) of sucrose

SUBMITTER: Grosz DD 

PROVIDER: S-EPMC3995326 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature