Genomic profiling reveals Pitx2 controls expression of mature extraocular muscle contraction-related genes.

ABSTRACT:

Purpose

To assess the influence of the Pitx2 transcription factor on the global gene expression profile of extraocular muscle (EOM) of mice.

Methods

Mice with a conditional knockout of Pitx2, designated Pitx2(Δflox/Δflox) and their control littermates Pitx2(flox/flox), were used. RNA was isolated from EOM obtained at 3, 6, and 12 weeks of age and processed for microarray-based profiling. Pairwise comparisons were performed between mice of the same age and differentially expressed gene lists were generated. Select genes from the profile were validated using real-time quantitative polymerase chain reaction and protein immunoblot. Ultrastructural analysis was performed to evaluate EOM sarcomeric structure.

Results

The number of differentially expressed genes was relative

SUBMITTER: Zhou Y 

PROVIDER: S-EPMC3995565 | biostudies-literature | 2012 Apr

REPOSITORIES: biostudies-literature