Large-scale determination of sequence, structure, and function relationships in cytosolic glutathione transferases across the biosphere.

ABSTRACT: The cytosolic glutathione transferase (cytGST) superfamily comprises more than 13,000 nonredundant sequences found throughout the biosphere. Their key roles in metabolism and defense against oxidative damage have led to thousands of studies over several decades. Despite this attention, little is known about the physiological reactions they catalyze and most of the substrates used to assay cytGSTs are synthetic compounds. A deeper understanding of relationships across the superfamily could provide new clues about their functions. To establish a foundation for expanded classification of cytGSTs, we generated similarity-based subgroupings for the entire superfamily. Using the resulting sequence similarity networks, we chose targets that broadly covered unknown functions and report here experi

SUBMITTER: Mashiyama ST 

PROVIDER: S-EPMC3995644 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature