Homogenous 96-plex PEA immunoassay exhibiting high sensitivity, specificity, and excellent scalability.

ABSTRACT: Medical research is developing an ever greater need for comprehensive high-quality data generation to realize the promises of personalized health care based on molecular biomarkers. The nucleic acid proximity-based methods proximity ligation and proximity extension assays have, with their dual reporters, shown potential to relieve the shortcomings of antibodies and their inherent cross-reactivity in multiplex protein quantification applications. The aim of the present study was to develop a robust 96-plex immunoassay based on the proximity extension assay (PEA) for improved high throughput detection of protein biomarkers. This was enabled by: (1) a modified design leading to a reduced number of pipetting steps compared to the existing PEA protocol, as well as improved intra-assay precision

SUBMITTER: Assarsson E 

PROVIDER: S-EPMC3995906 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature