Whole-mount MeFISH: a novel technique for simultaneous visualization of specific DNA methylation and protein/RNA expression.

ABSTRACT: To understand the spatiotemporal changes in cellular status that occur during embryonic development, it is desirable to detect simultaneously the expression of genes, proteins, and epigenetic modifications in individual embryonic cells. A technique termed methylation-specific fluorescence in situ hybridization (MeFISH) was developed recently that can visualize the methylation status of specific DNA sequences in cells fixed on a glass slide. Here, we adapted this glass slide-based MeFISH to the study of intact embryos, and established a method called whole-mount MeFISH. This method can be applied to any DNA sequences in theory and, as a proof-of-concept experiment, we examined the DNA methylation status of satellite repeats in developing mouse primordial germ cells, in which global DNA deme

SUBMITTER: Shiura H 

PROVIDER: S-EPMC3995954 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature