An automated integrated platform for rapid and sensitive multiplexed protein profiling using human saliva samples.

ABSTRACT: During the last decade, saliva has emerged as a potentially ideal diagnostic biofluid for noninvasive testing. In this paper, we present an automated, integrated platform useable by minimally trained personnel in the field for the diagnosis of respiratory diseases using human saliva as a sample specimen. In this platform, a saliva sample is loaded onto a disposable microfluidic chip containing all the necessary reagents and components required for saliva analysis. The chip is then inserted into the automated analyzer, the SDReader, where multiple potential protein biomarkers for respiratory diseases are measured simultaneously using a microsphere-based array via fluorescence sandwich immunoassays. The results are read optically, and the images are analyzed by a custom-designed algorithm. T

SUBMITTER: Nie S 

PROVIDER: S-EPMC3996899 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature