Quantitative analysis of colony morphology in yeast.

ABSTRACT: Microorganisms often form multicellular structures such as biofilms and structured colonies that can influence the organism's virulence, drug resistance, and adherence to medical devices. Phenotypic classification of these structures has traditionally relied on qualitative scoring systems that limit detailed phenotypic comparisons between strains. Automated imaging and quantitative analysis have the potential to improve the speed and accuracy of experiments designed to study the genetic and molecular networks underlying different morphological traits. For this reason, we have developed a platform that uses automated image analysis and pattern recognition to quantify phenotypic signatures of yeast colonies. Our strategy enables quantitative analysis of individual colonies, measured at a sin

SUBMITTER: Ruusuvuori P 

PROVIDER: S-EPMC3996921 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature