Prediction and analysis of canonical EF hand loop and qualitative estimation of Ca²⁺ binding affinity.

ABSTRACT: The diversity of functions carried out by EF hand-containing calcium-binding proteins is due to various interactions made by these proteins as well as the range of affinity levels for Ca²⁺ displayed by them. However, accurate methods are not available for prediction of binding affinities. Here, amino acid patterns of canonical EF hand sequences obtained from available crystal structures were used to develop a classifier that distinguishes Ca²⁺-binding loops and non Ca²⁺-binding regions with 100% accuracy. To investigate further, we performed a proteome-wide prediction for E. histolytica, and classified known EF-hand proteins. We compared our results with published methods on the E. histolytica proteome scan, and demonstrated our method to be more specific and accurate for predicting potent

SUBMITTER: Mazumder M 

PROVIDER: S-EPMC3997525 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature