Use of IgE and IgG4 epitope binding to predict the outcome of oral immunotherapy in cow's milk allergy.

ABSTRACT:

Background

Oral immunotherapy (OIT) with cow's milk (CM) has been reported to induce a number of specific antibody responses, but these remain to be fully characterized. Our objective was to explore whether IgE and IgG4 epitope binding profiles could predict the risk of side effects during CM OIT.

Methods

The study population consisted of 32 children (6-17 yr of age) with CM allergy: 26 children who successfully completed OIT and six children who discontinued therapy due to adverse reactions. We investigated sera drawn before and after OIT. We analyzed specific IgE and IgG4 binding to CM protein-derived peptides with a microarray-based immunoassay. Antibody binding affinity was analyzed with a competition assay where CM proteins in solution competed with peptides printed on

SUBMITTER: Savilahti EM 

PROVIDER: S-EPMC3997594 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature