Invertebrate specific D1-like dopamine receptor in control of salivary glands in the black-legged tick Ixodes scapularis.

ABSTRACT: The control of tick salivary secretion, which plays a crucial role in compromising the host immune system, involves complex neural mechanisms. Dopamine is known to be the most potent activator of salivary secretion, as a paracrine/autocrine factor. We describe the invertebrate-specific D1-like dopamine receptor (InvD1L), which is highly expressed in tick salivary glands. The InvD1L phylogenic clade was found only in invertebrates, suggesting that this receptor was lost in vertebrates during evolution. InvD1L expressed in Chinese hamster ovary (CHO)-K1 cells was activated by dopamine with a median effective dose (EC50 ) of 1.34 μM. Immunohistochemistry using the antibody raised against InvD1L revealed two different types of immunoreactivities: basally located axon terminals that are colocal

SUBMITTER: Simo L 

PROVIDER: S-EPMC3997621 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature