Characterization of TgPuf1, a member of the Puf family RNA-binding proteins from Toxoplasma gondii.

ABSTRACT:

Background

Puf proteins act as translational regulators and affect many cellular processes in a wide range of eukaryotic organisms. Although Puf proteins have been well characterized in many model systems, little is known about the structural and functional characteristics of Puf proteins in the parasite Toxoplasma gondii.

Methods

Using a combination of conventional molecular approaches, we generated endogenous TgPuf1 tagged with hemagglutinin (HA) epitope and investigated the TgPuf1 expression levels and localization in the tachyzoites and bradyzoites. We used RNA Electrophoretic Mobility Shfit Assay (EMSA) to determine whether the recombination TgPuf1 has conserverd RNA binding activity and specificity.

Results

TgPuf1 was expressed at a significantly higher level in

SUBMITTER: Liu M 

PROVIDER: S-EPMC3997814 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature