NeuroArray: a universal interface for patterning and interrogating neural circuitry with single cell resolution.

ABSTRACT: Recreation of neural network in vitro with designed topology is a valuable tool to decipher how neurons behave when interacting in hierarchical networks. In this study, we developed a simple and effective platform to pattern primary neurons in array formats for interrogation of neural circuitry with single cell resolution. Unlike many surface-chemistry-based patterning methods, our NeuroArray technique is specially designed to accommodate neuron's polarized morphologies to make regular arrays of cells without restricting their neurite outgrowth, and thus allows formation of freely designed, well-connected, and spontaneously active neural network. The NeuroArray device was based on a stencil design fabricated using a novel sacrificial-layer-protected PDMS molding method that enables product

SUBMITTER: Li W 

PROVIDER: S-EPMC3998032 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature