Automated high-throughput quantification of mitotic spindle positioning from DIC movies of Caenorhabditis embryos.

ABSTRACT: The mitotic spindle is a microtubule-based structure that elongates to accurately segregate chromosomes during anaphase. Its position within the cell also dictates the future cell cleavage plan, thereby determining daughter cell orientation within a tissue or cell fate adoption for polarized cells. Therefore, the mitotic spindle ensures at the same time proper cell division and developmental precision. Consequently, spindle dynamics is the matter of intensive research. Among the different cellular models that have been explored, the one-cell stage C. elegans embryo has been an essential and powerful system to dissect the molecular and biophysical basis of spindle elongation and positioning. Indeed, in this large and transparent cell, spindle poles (or centrosomes) can be easily detected fr

SUBMITTER: Cluet D 

PROVIDER: S-EPMC3998942 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature