Investigating the reactivities of a polyketide synthase module through fluorescent click chemistry.

ABSTRACT: A method for monitoring in vitro polyketide synthesis has been developed whereby nonchromophoric polyketide products are made brightly fluorescent in a simple, rapid, inexpensive, and bioorthogonal manner through CuAAC with a sulforhodamine B azide derivative.

SUBMITTER: Hughes AJ 

PROVIDER: S-EPMC3999232 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature