Altered gamma-aminobutyric acid type B receptor subunit 1 splicing in alcoholics.

ABSTRACT:

Background

Chronic alcohol exposure can change splice variant expression. The gamma-aminobutyric acid type B (GABAB) receptor undergoes splicing and is an alcoholism treatment target, but there is little information about splicing changes in this receptor in alcoholics. We studied GABAB receptor subunit 1 (GABAB1) splicing in alcoholic postmortem brains.

Methods

To maximize GABAB1 splice junction identification, we combined gene specific libraries with RNA-seq. Splice junctions and mapped reads were also found from intronic and intergenic regions. We compared GABAB1 splice junctions in prefrontal cortices from 14 alcoholic and 15 control subjects and introduced new strategies, reads per kilobase of splice junction model per million mapped reads and reads per kilobase of gene

SUBMITTER: Lee C 

PROVIDER: S-EPMC3999301 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature