Predicting HIV-1 broadly neutralizing antibody epitope networks using neutralization titers and a novel computational method.

ABSTRACT:

Background

Recent efforts in HIV-1 vaccine design have focused on immunogens that evoke potent neutralizing antibody responses to a broad spectrum of viruses circulating worldwide. However, the development of effective vaccines will depend on the identification and characterization of the neutralizing antibodies and their epitopes. We developed bioinformatics methods to predict epitope networks and antigenic determinants using structural information, as well as corresponding genotypes and phenotypes generated by a highly sensitive and reproducible neutralization assay.282 clonal envelope sequences from a multiclade panel of HIV-1 viruses were tested in viral neutralization assays with an array of broadly neutralizing monoclonal antibodies (mAbs: b12, PG9,16, PGT121 - 128, PGT130 -

SUBMITTER: Evans MC 

PROVIDER: S-EPMC3999910 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature