Rejuvenation of human cardiac progenitor cells with Pim-1 kinase.

ABSTRACT:

Rationale

Myocardial function is enhanced by adoptive transfer of human cardiac progenitor cells (hCPCs) into a pathologically challenged heart. However, advanced age, comorbidities, and myocardial injury in patients with heart failure constrain the proliferation, survival, and regenerative capacity of hCPCs. Rejuvenation of senescent hCPCs will improve the outcome of regenerative therapy for a substantial patient population possessing functionally impaired stem cells.

Objective

Reverse phenotypic and functional senescence of hCPCs by ex vivo modification with Pim-1.

Methods and results

C-kit-positive hCPCs were isolated from heart biopsy samples of patients undergoing left ventricular assist device implantation. Growth kinetics, telomere lengths, and expression of ce

SUBMITTER: Mohsin S 

PROVIDER: S-EPMC3999968 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature