Genetic diversity of rotavirus genome segment 6 (encoding VP6) in Pretoria, South Africa.

ABSTRACT:

Background

Rotavirus viral protein 6 (VP6), encoded by genome segment (GS) 6, is the primary target for rotavirus diagnosis by serological and some molecular techniques. Selected full length nucleotide sequences of GS 6 of rotavirus strains from South Africa were sequenced and analysed to determine genetic diversity and variations within the circulating rotaviruses.

Findings

The VP6 amplicons were sequenced using the Sanger ABI 3130xl. Phylogenetic and pairwise analysis revealed that the VP6 genes of the study strains belonged to two different VP6 [I] genotypes. Five sequences were assigned genotype I1 and seven as genotype I2. Comparison of the group specific antigenic regions of the South African strains to the reference strains, shows that the South African VP6 sequences

SUBMITTER: Nyaga MM 

PROVIDER: S-EPMC4000354 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature