Influence of DNA extraction on oral microbial profiles obtained via 16S rRNA gene sequencing.

ABSTRACT: The advent of next-generation sequencing has significantly facilitated characterization of the oral microbiome. Despite great efforts in streamlining the processes of sequencing and data curation, upstream steps required for amplicon library generation could still influence 16S rRNA gene-based microbial profiles. Among upstream processes, DNA extraction is a critical step that could represent a great source of bias. Accounting for bias introduced by extraction procedures is important when comparing studies that use different methods. Identifying the method that best portrays communities is also desirable. Accordingly, the aim of this study was to evaluate bias introduced by different DNA extraction procedures on oral microbiome profiles. Four DNA extraction methods were tested on mock comm

SUBMITTER: Abusleme L 

PROVIDER: S-EPMC4000428 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature