Improved isobaric tandem mass tag quantification by ion mobility mass spectrometry.

ABSTRACT:

Rationale

Isobaric tandem mass tags are an attractive alternative to mass difference tags and label-free approaches for quantitative proteomics due to the high degree of multiplexing that can be performed with their implementation. A drawback of tandem mass tags are that the co-isolation and co-fragmentation of labeled peptide precursors can result in chimeric tandem mass (MS/MS) spectra that can underestimate the fold-change expression of each peptide. Ion mobility (IM) separations coupled to quadrupole time-of-flight (Q-TOF) instruments have the potential to mitigate MS/MS spectra chimeracy since IM-MS has the ability to separate ions based on charge, m/z, and collision cross section (CCS).

Methods

Two complex protein mixtures, labeled with DiLeu isobaric tandem mass tags

SUBMITTER: Sturm RM 

PROVIDER: S-EPMC4000571 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature