Retinal expression of the serine protease matriptase-2 (Tmprss6) and its role in retinal iron homeostasis.

ABSTRACT:

Purpose

Matriptase-2 (also known as TMPRSS6) is a critical regulator of the iron-regulatory hormone hepcidin in the liver; matriptase-2 cleaves membrane-bound hemojuvelin and consequently alters bone morphogenetic protein (BMP) signaling. Hemojuvelin and hepcidin are expressed in the retina and play a critical role in retinal iron homeostasis. However, no information on the expression and function of matriptase-2 in the retina is available. The purpose of the present study was to examine the retinal expression of matriptase-2 and its role in retinal iron homeostasis.

Methods

RT-PCR, quantitative PCR (qPCR), and immunofluorescence were used to analyze the expression of matriptase-2 and other iron-regulatory proteins in the mouse retina. Polarized localization of matriptase-2

SUBMITTER: Gnana-Prakasam JP 

PROVIDER: S-EPMC4000719 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature