Evaluation of insulin medium or chondrogenic medium on proliferation and chondrogenesis of ATDC5 cells.

ABSTRACT:

Background

The ATDC5 cell line is regarded as an excellent cell model for chondrogenesis. In most studies with ATDC5 cells, insulin medium (IM) was used to induce chondrogenesis while chondrogenic medium (CM), which was usually applied in chondrogenesis of mesenchymal stem cells (MSCs), was rarely used for ATDC5 cells. This study was mainly designed to investigate the effect of IM, CM, and growth medium (GM) on chondrogenesis of ATDC5 cells.

Methods

ATDC5 cells were, respectively, cultured in IM, CM, and GM for a certain time. Then the proliferation and the chondrogenesis progress of cells in these groups were analyzed.

Results

Compared with CM and GM, IM promoted the proliferation of cells significantly. CM was effective for enhancement of cartilage specific markers,

SUBMITTER: Yao Y 

PROVIDER: S-EPMC4000943 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature