Rapid bacterial whole-genome sequencing to enhance diagnostic and public health microbiology.

ABSTRACT:

Importance

The latest generation of benchtop DNA sequencing platforms can provide an accurate whole-genome sequence (WGS) for a broad range of bacteria in less than a day. These could be used to more effectively contain the spread of multidrug-resistant pathogens.

Objective

To compare WGS with standard clinical microbiology practice for the investigation of nosocomial outbreaks caused by multidrug-resistant bacteria, the identification of genetic determinants of antimicrobial resistance, and typing of other clinically important pathogens.

Design, setting, and participants

A laboratory-based study of hospital inpatients with a range of bacterial infections at Cambridge University Hospitals NHS Foundation Trust, a secondary and tertiary referral center in England, compa

SUBMITTER: Reuter S 

PROVIDER: S-EPMC4001082 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature