ResLog plots as an empirical metric of the quality of cryo-EM reconstructions.

ABSTRACT: Compared to the field of X-ray crystallography, the field of single particle three-dimensional electron microscopy has few reliable metrics for assessing the quality of 3D reconstructions. New metrics are needed that can determine whether a given 3D reconstruction accurately reflects the structure of the particles from which it was derived or instead depicts a plausible though incorrect structure due to coarse misalignment of particles. Here an empirical procedure is presented for differentiating between a reconstruction with well-aligned particles and a reconstruction with grossly misclassified particles. For a given dataset, 3D reconstructions are computed from subsets of particles with decreasing numbers of particles contributing to the reconstruction. A plot of inverse resolution vs. t

SUBMITTER: Stagg SM 

PROVIDER: S-EPMC4001718 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature