Cell-selective labeling using amino acid precursors for proteomic studies of multicellular environments.

ABSTRACT: We report a technique to selectively and continuously label the proteomes of individual cell types in coculture, named cell type-specific labeling using amino acid precursors (CTAP). Through transgenic expression of exogenous amino acid biosynthesis enzymes, vertebrate cells overcome their dependence on supplemented essential amino acids and can be selectively labeled through metabolic incorporation of amino acids produced from heavy isotope-labeled precursors. When testing CTAP in several human and mouse cell lines, we could differentially label the proteomes of distinct cell populations in coculture and determine the relative expression of proteins by quantitative mass spectrometry. In addition, using CTAP we identified the cell of origin of extracellular proteins secreted from cells in

SUBMITTER: Gauthier NP 

PROVIDER: S-EPMC4002004 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature