Microparticles released by Listeria monocytogenes-infected macrophages are required for dendritic cell-elicited protective immunity.

ABSTRACT: Interplay between macrophages and dendritic cells in the processing and presentation of bacterial antigens for T-cell immune responses remains poorly understood. Using a Listeria monocytogenes (Lm) infection model, we demonstrate that dendritic cells (DCs) require the support of macrophages to elicit protective immunity against Lm infection. DCs themselves were inefficient at taking up Lm but capable of taking up microparticles (MPs) released by Lm-infected macrophages. These MPs transferred Lm antigens to DCs, allowing DCs to present Lm antigen to effector T cells. MP-mediated Lm antigen transfer required MHC class I participation, since MHC class I deficiency in macrophages resulted in a significant reduction of T-cell activation. Moreover, the vaccination of mice with MPs from Lm-infect

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC4002214 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature