Berbamine exhibits potent antitumor effects on imatinib-resistant CML cells in vitro and in vivo.

ABSTRACT:

Aim

The aim of this study was to explore the effects and mechanism of berbamine on imatinib-resistant BCR-ABL-positive human leukemia K562 (K562-r) cells in vitro and in vivo.

Methods

Cell viability was measured by MTT assay, and apoptotic morphology changes were detected by fluorescence microscopy. The apoptosis rate was measured by flow cytometric assay. mdr-1 mRNA levels were determined by RT-PCR. Bcl-2 family proteins, cytochrome c(cyt C), poly (ADP-ribose) polymerase (PARP), and P-glycoprotein were detected by Western blot. BALB/c nu/nu mice were injected with K562-r cells subcutaneously. Tumor-bearing mice were treated intravenously with berbamine.

Results

MTT tests revealed that berbamine significantly inhibited K562-r cell proliferation and increased the chemo

SUBMITTER: Wei YL 

PROVIDER: S-EPMC4002272 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature