Oligomannurarate sulfate blocks tumor growth by inhibiting NF-kappaB activation.

ABSTRACT:

Aim

JG3, a novel marine-derived oligosaccharide, significantly inhibits angiogenesis and tumor metastasis by blocking heparanase activity. It also arrests tumor growth, an effect that is not fully explained by its anti-heparanase activity. Here we sought to identify the mechanisms underlying JG3-mediated inhibition of tumor growth.

Methods

Heparanase expression was assessed by RT-PCR and Western blotting. NF-kappaB activation status was determined using immunofluorescence, Western blotting, DNA-binding and transcription-activity assays. The effect of JG3 on upstream components of the NF-kappaB pathway and on selected transcription factors were monitored by Western blotting. The antitumor effect of JG3 and its relation to NF-kappaB activation were evaluated using four differe

SUBMITTER: Zhang J 

PROVIDER: S-EPMC4002413 | biostudies-literature | 2010 Mar

REPOSITORIES: biostudies-literature