Proteomic analysis of the effect of iptakalim on human pulmonary arterial smooth muscle cell proliferation.

ABSTRACT:

Aim

To investigate the anti-proliferative effect of iptakalim (Ipt), a newly selective K(ATP) channel opener, in endothelin-1 (ET-1)-induced human pulmonary arterial smooth muscle cells (PASMCs) using proteomic analysis.

Methods

Human PASMCs were incubated with ET-1 (10(-8) mol/L) and ET-1 (10(-8) mol/L) plus iptaklim (10(-5) mol/L) for 24 h. Analysis via 2-DE gel electrophoresis and MALDI-TOF-MS was employed to display the different protein profiles of whole-cell protein from cultures of control, ET-1 treatment alone, and treatment with ET-1 and iptaklim combined. Real time RT-PCR and Western blot analysis were used to confirm the proteomic analysis.

Results

When iptakalim inhibited the proliferative effect of ET-1 in human PASMCs by opening the K(ATP) channels, the

SUBMITTER: Yang MX 

PROVIDER: S-EPMC4002473 | biostudies-literature | 2009 Feb

REPOSITORIES: biostudies-literature