Aspirin inhibits proliferation of gemcitabine-resistant human pancreatic cancer cells and augments gemcitabine-induced cytotoxicity.

ABSTRACT:

Aim

To investigate whether aspirin is able to augment gemcitabine-induced cytotoxicity in human pancreatic cancer cells.

Methods

Two gemcitabine-insensitive human pancreatic cancer cell lines, PANC-1 and Capan-1, were used. Cells were treated with either aspirin or gemcitabine alone or both of them. Cell growth and apoptosis were determined by MTT assay, Annexin V or Hoechest 33258 staining. Cell cycle distribution was examined by flow cytometry. Western blot with specific phosphorylated protein antibodies was used to detect the activation of protein kinase. RT-PCR and Western blot were applied to assess the transcription and protein level for cyclin D1 and Bcl-2.

Results

Aspirin alone significantly inhibits the proliferation of PANC-1 cells by causing cell cycle arre

SUBMITTER: Ou YQ 

PROVIDER: S-EPMC4002689 | biostudies-literature | 2010 Jan

REPOSITORIES: biostudies-literature