Inhibition of Hec1 expression enhances the sensitivity of human ovarian cancer cells to paclitaxel.

ABSTRACT:

Aim

Hec1, a member of the Ndc80 kinetochore complex, is highly expressed in cancers. The aim of this study was to explore the role and mechanism of action of Hec1 with respect to the cytotoxicity of paclitaxel in ovarian cancer.

Methods

Thirty ovarian cancer samples and 6 normal ovarian samples were collected. Hec1 expression in these samples was determined with immunohistochemistry. Ovarian cancer cell lines A2780, OV2008, C13K, SKOV3, and CAOV3 and A2780/Taxol were examined. Cell apoptosis and cell cycle analysis were detected with flow cytometric technique. siRNA was used to delete Hec1 in the cells. The expression of related mRNAs and proteins was measured using RT-PCR and Western blot analysis, respectively.

Results

Hec1 expression was significantly higher in ova

SUBMITTER: Mo QQ 

PROVIDER: S-EPMC4002790 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature