Response of mesenchymal stem cells to shear stress in tissue-engineered vascular grafts.

ABSTRACT:

Aim

Recent studies have demonstrated that mesenchymal stem cells (MSCs) can differentiate into endothelial cells. The effect of shear stress on MSC differentiation is incompletely understood, and most studies have been based on two-dimensional systems. We used a model of tissue-engineered vascular grafts (TEVGs) to investigate the effects of shear stress on MSC differentiation.

Methods

MSCs were isolated from canine bone marrow. The TEVG was constructed by seeding MSCs onto poly-epsilon-caprolactone and lactic acid (PCLA) scaffolds and subjecting them to shear stress provided by a pulsatile bioreactor for four days (two days at 1 dyne/cm(2) to 15 dyne/cm(2) and two days at 15 dyne/cm(2)).

Results

Shear stress significantly increased the expression of endothelial cell

SUBMITTER: Dong JD 

PROVIDER: S-EPMC4002825 | biostudies-literature | 2009 May

REPOSITORIES: biostudies-literature