Immunomodulatory activity of andrographolide on macrophage activation and specific antibody response.

ABSTRACT:

Aim

To investigate the immunomodulatory effects of andrographolide on both innate and adaptive immune responses.

Methods

Andrographolide (10 microg/mL in vitro or 1 mg/kg in vivo) was used to modulate LPS-induced classical activated (M1) or IL-4-induced alternative activated (M2) macrophages in vitro and humor immune response to HBsAg in vivo. Cytokine gene expression profile (M1 vs M2) was measured by real-time PCR, IL-12/IL-10 level was detected by ELISA, and surface antigen expression was evaluated by flow cytometry, whereas phosphorylation level of ERK 1/2 and AKT was determined by Western blot. The level of anti-HBs antibodies in HBsAg immunized mice was detected by ELISA, and the number of HBsAg specific IL-4-producing splenocyte was enumerated by ELISPOT.

Results</

SUBMITTER: Wang W 

PROVIDER: S-EPMC4002847 | biostudies-literature | 2010 Feb

REPOSITORIES: biostudies-literature