Talin-bound NPLY motif recruits integrin-signaling adapters to regulate cell spreading and mechanosensing.

ABSTRACT: Integrin-dependent cell adhesion and spreading are critical for morphogenesis, tissue regeneration, and immune defense but also tumor growth. However, the mechanisms that induce integrin-mediated cell spreading and provide mechanosensing on different extracellular matrix conditions are not fully understood. By expressing β3-GFP-integrins with enhanced talin-binding affinity, we experimentally uncoupled integrin activation, clustering, and substrate binding from its function in cell spreading. Mutational analysis revealed Tyr747, located in the first cytoplasmic NPLY(747) motif, to induce spreading and paxillin adapter recruitment to substrate- and talin-bound integrins. In addition, integrin-mediated spreading, but not focal adhesion localization, was affected by mutating adjacent sequence

SUBMITTER: Pinon P 

PROVIDER: S-EPMC4003243 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature