Proteome reference map and regulation network of neonatal rat cardiomyocyte.

ABSTRACT:

Aim

To study and establish a proteome reference map and regulation network of neonatal rat cardiomyocyte.

Methods

Cultured cardiomyocytes of neonatal rats were used. All proteins expressed in the cardiomyocytes were separated and identified by two-dimensional polyacrylamide gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS). Biological networks and pathways of the neonatal rat cardiomyocytes were analyzed using the Ingenuity Pathway Analysis (IPA) program (www.ingenuity.com). A 2-DE database was made accessible on-line by Make2ddb package on a web server.

Results

More than 1000 proteins were separated on 2D gels, and 148 proteins were identified. The identified proteins were used for the construct

SUBMITTER: Li ZJ 

PROVIDER: S-EPMC4003303 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature