Implantation of bFGF-treated islet progenitor cells ameliorates streptozotocin-induced diabetes in rats.

ABSTRACT:

Aim

To examine whether implantation of islet preparation-derived proliferating islet cells (PIC) could ameliorate diabetes in rats.

Methods

PIC were expanded from rat islet preparation by supplementation of basic fibroblast growth factor (bFGF) and implanted into rats with streptozotocin (STZ)-induced diabetes through the portal vein. Body weight and blood glucose levels were measured. Serum insulin levels were measured by radioimmunoassay. The presence of insulin-positive cells was determined by hematoxylin and immunohistochemical staining.

Results

Cultured islet cells (CIC) were demonstrated to dedifferentiate in vitro, and the apoptosis ratios reached more than 50% by the 15th day post-isolation. PIC cells treated with bFGF (20 ng/mL) continued growing within 30 da

SUBMITTER: Li G 

PROVIDER: S-EPMC4003334 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature