Large-scale microsatellite development in grasspea (Lathyrus sativus L.), an orphan legume of the arid areas.

ABSTRACT:

Background

Grasspea (Lathyrus sativus L., 2n = 14), a member of the family Leguminosae, holds great agronomic potential as grain and forage legume crop in the arid areas for its superb resilience to abiotic stresses such as drought, flood and salinity. The crop could not make much progress through conventional breeding in the past, and there are hardly any detailed molecular biology studies due to paucity of reliable molecular markers representative of the entire genome.

Results

Using the 454 FLX Titanium pyrosequencing technique, 651,827 simple sequence repeat (SSR) loci were identified and 50,144 nonredundant primer pairs were successfully designed, of which 288 were randomly selected for validation among 23 L. sativus and one L. cicera accessions of diverse provenance. 74

SUBMITTER: Yang T 

PROVIDER: S-EPMC4003855 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature