Identification of mycoparasitism-related genes against the phytopathogen Sclerotinia sclerotiorum through transcriptome and expression profile analysis in Trichoderma harzianum.

ABSTRACT:

Background

The species of T. harzianum are well known for their biocontrol activity against plant pathogens. However, few studies have been conducted to further our understanding of its role as a biological control agent against S. sclerotiorum, a pathogen involved in several crop diseases around the world. In this study, we have used RNA-seq and quantitative real-time PCR (RT-qPCR) techniques in order to explore changes in T. harzianum gene expression during growth on cell wall of S. sclerotiorum (SSCW) or glucose. RT-qPCR was also used to examine genes potentially involved in biocontrol, during confrontation between T. harzianum and S. sclerotiorum.

Results

Data obtained from six RNA-seq libraries were aligned onto the T. harzianum CBS 226.95 reference genome and compared

SUBMITTER: Steindorff AS 

PROVIDER: S-EPMC4004048 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature