Rapid detection of Plasmodium falciparum with isothermal recombinase polymerase amplification and lateral flow analysis.

ABSTRACT:

Background

Nucleic acid amplification is the most sensitive and specific method to detect Plasmodium falciparum. However the polymerase chain reaction remains laboratory-based and has to be conducted by trained personnel. Furthermore, the power dependency for the thermocycling process and the costly equipment necessary for the read-out are difficult to cover in resource-limited settings. This study aims to develop and evaluate a combination of isothermal nucleic acid amplification and simple lateral flow dipstick detection of the malaria parasite for point-of-care testing.

Methods

A specific fragment of the 18S rRNA gene of P. falciparum was amplified in 10 min at a constant 38°C using the isothermal recombinase polymerase amplification (RPA) method. With a unique probe syst

SUBMITTER: Kersting S 

PROVIDER: S-EPMC4004163 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature