Proximity interactions among centrosome components identify regulators of centriole duplication.

ABSTRACT: The centrosome consists of a pair of centrioles and surrounding pericentriolar material (PCM). Many vertebrate cells also have an array of granules, termed centriolar satellites, that localize around the centrosome and are associated with centrosome and cilium function. Centriole duplication occurs once per cell cycle and is effected by a set of proteins including PLK4, CEP192, CEP152, CEP63, and CPAP. Information on the relationships between these components is limited due to the difficulty in assaying interactions in the context of the centrosome. Here, we used proximity-dependent biotin identification (BioID) to identify proximity interactions among centriole duplication proteins. PLK4, CEP192, and CEP152 BioID identified known physically interacting proteins and a new interaction betwe

SUBMITTER: Firat-Karalar EN 

PROVIDER: S-EPMC4004176 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature