A step closer to membrane protein multiplexed nanoarrays using biotin-doped polypyrrole.

ABSTRACT: Whether for fundamental biological research or for diagnostic and drug discovery applications, protein micro- and nanoarrays are attractive technologies because of their low sample consumption, high-throughput, and multiplexing capabilities. However, the arraying platforms developed so far are still not able to handle membrane proteins, and specific methods to selectively immobilize these hydrophobic and fragile molecules are needed to understand their function and structural complexity. Here we integrate two technologies, electropolymerization and amphipols, to demonstrate the electrically addressable functionalization of micro- and nanosurfaces with membrane proteins. Gold surfaces are selectively modified by electrogeneration of a polymeric film in the presence of biotin, where avidin c

SUBMITTER: Della Pia EA 

PROVIDER: S-EPMC4004317 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature