Comparison of 4 direct Coombs' test methods with polyclonal antiglobulins in anemic and nonanemic dogs for in-clinic or laboratory use.

ABSTRACT:

Background

Difficulties with the direct antiglobulin test (DAT) and its apparent lack of sensitivity and specificity for immune-mediated hemolytic anemia (IMHA) in dogs have raised skepticism regarding its diagnostic value.

Objective

To compare different DATs and other hematologic parameters in dogs.

Animals

Anticoagulated blood samples from 59 nonanemic and 46 anemic dogs (± IMHA) from a research colony and veterinary clinics.

Methods

Prospective observational study: Immunochromatographic strip, gel microcolumn, and capillary techniques were compared with standard microtiter DAT using 2 polyvalent antiglobulins. Spherocytosis, autoagglutination, osmotic fragility, and clinical data were assessed.

Results

Blood samples from all 59 nonanemic dogs were DAT

SUBMITTER: Caviezel LL 

PROVIDER: S-EPMC4004353 | biostudies-literature | 2014 Mar-Apr

REPOSITORIES: biostudies-literature