A flow cytometric assay to quantify invasion of red blood cells by rodent Plasmodium parasites in vivo.

ABSTRACT:

Background

Malaria treatments are becoming less effective due to the rapid spread of drug resistant parasites. Increased understanding of the host/parasite interaction is crucial in order to develop treatments that will be less prone to resistance. Parasite invasion of the red blood cell (RBC) is a critical aspect of the parasite life cycle and is, therefore, a promising target for the development of malaria treatments. Assays for analysing parasite invasion in vitro have been developed, but no equivalent assays exist for in vivo studies. This article describes a novel flow cytometric in vivo parasite invasion assay.

Methods

Experiments were conducted with mice infected with erythrocytic stages of Plasmodium chabaudi adami strain DS. Exogenously labelled blood cells were tra

SUBMITTER: Lelliott PM 

PROVIDER: S-EPMC4004390 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature